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| Galler,R.; Marchevsky,R.S.; Caride,E.; Almeida,L.F.C.; Yamamura,A.M.Y.; Jabor,A.V.; Motta,M.C.A.; Bonaldo,M.C.; Coutinho,E.S.F.; Freire,M.S.. |
| A chimeric yellow fever (YF)-dengue serotype 2 (dengue 2) virus was constructed by replacing the premembrane and envelope genes of the YF 17D virus with those from dengue 2 virus strains of Southeast Asian genotype. The virus grew to high titers in Vero cells and, after passage 2, was used for immunogenicity and attenuation studies in rhesus monkeys. Subcutaneous immunization of naive rhesus monkeys with the 17D-D2 chimeric virus induced a neutralizing antibody response associated with the protection of 6 of 7 monkeys against viremia by wild-type dengue 2 virus. Neutralizing antibody titers to dengue 2 were significantly lower in YF-immune animals than in YF-naive monkeys and protection against challenge with wild-type dengue 2 virus was observed in only 2... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: YF 17D virus recombinants; Dengue vaccine; Immunogenicity; Attenuation; Rhesus monkeys. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200012 |
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| Miyasato,PA; Ramos,CRR; Abreu,PAE; Dias,WO; Nascimento,C; Ho,PL; Kawano,T. |
| Schistosomes utilize proteinases to accomplish several activities such as tissue penetration, tissue digestion and evasion of host immune responses. Cathepsin L is a cysteine proteinase of the papain superfamily detected in their gut lumen which indicates that this enzyme contributes to the proteolysis of ingested hemoglobin. Due to the roles played in the schistosome biology, proteolytic enzymes are considered potential targets for developing and guiding antischistosomal therapies. In the present work, the cathepsin L1 cDNA coding of Schistosoma mansoni was cloned into the pAE vector that provides high-level expression of heterologous proteins in Escherichia coli. The recombinant protein was expressed as inclusion bodies, purified under denaturing... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Schistosoma mansoni; Cysteine proteinase; Cathepsin L1; Vaccine; Immunogenicity. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200011 |
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| Zerbini,Cristiano A.F.; Ribeiro dos Santos,Rodrigo; Jose Nunes,Maria; Soni,Jyoti; Li,Ping; Jain,Varsha K.; Ofori-Anyinam,Opokua. |
| Abstract The World Health Organization influenza forecast now includes an influenza B strain from each of the influenza B lineages (B/Yamagata and B/Victoria) for inclusion in seasonal influenza vaccines. Traditional trivalent influenza vaccines include an influenza B strain from one lineage, but because two influenza B lineages frequently co-circulate, the effectiveness of trivalent vaccines may be reduced in seasons of influenza B vaccine-mismatch. Thus, quadrivalent vaccines may potentially reduce the burden of influenza compared with trivalent vaccines.In this Phase III, open-label study, we assessed the immunogenicity and safety of Southern Hemisphere inactivated quadrivalent influenza vaccine (Fluarix™ Tetra) in Brazilian adults (NCT02369341). The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Quadrivalent; Influenza; Vaccine; Immunogenicity; Safety; Brazil; Adults. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000100063 |
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| Valenzuela B.,M. Teresa; Altuzarra H.,Rogelio; Trucco A.,Olivia; Villegas R.,Rodrigo; Inostroza S.,Jaime; Granata S.,Paulo; Fleiderman V.,José; Maggi C.,Leonardo. |
| S. pneumoniae is a significant cause of community-acquired pneumonia in the elderly, and accounts for the majority of the pneumonia deaths among the elderly. We conducted this randomized double-blind study to evaluate the immune response to a 23-valent pneumococcal polysaccharide vaccine and the persistence of antibodies two years after the vaccination in an elderly population in Santiago, Chile. A total of 118 elderly nursing home residents received either the pneumococcal or a tetanus control vaccine. Serum samples were taken at enrolment, at two months, and at two years post-vaccination. Pre-vaccination anti-pneumococcal antibody geometric mean concentrations (GMC) were similar in both study groups, with increased levels of antibodies found only against... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Streptococcus pneumoniae; Vaccine; Elderly; Immunogenicity; Antibodies. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000300005 |
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| Mohabatkar,H.; Kar,S.K.. |
| We describe the impact of subtype differences on the seroreactivity of linear antigenic epitopes in envelope glycoprotein of HIV-1 isolates from different geographical locations. By computer analysis, we predicted potential antigenic sites of envelope glycoprotein (gp120 and gp4l) of this virus. For this purpose, after fetching sequences of proteins of interest from data banks, values of hydrophilicity, flexibility, accessibility, inverted hydrophobicity, and secondary structure were considered. We identified several potential antigenic epitopes in a B subtype strain of envelope glycoprotein of HIV-1 (IIIB). Solid- phase peptide synthesis methods of Merrifield and Fmoc chemistry were used for synthesizing peptides. These synthetic peptides corresponded... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Human immunodeficiency virus; Glycoprotein; Epitope; Immunogenicity. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500008 |
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| Zhang,Li; Liu,Dan; Pu,Dan; Wang,Yanwen; Li,Li; He,Yanqi; Li,Yalun; Li,Lei; Li,Weimin. |
| BACKGROUND: Mesenchymal stem cells (MSCs) are considered the best candidate in stem cells therapy due to their multipotent differentiation ability, low expression of co-stimulatory molecules (CD80, CD86, CD34 and HLA-II) and immunosuppression effects on in vivo immune responses. MSCs were now widely used in clinical trials but received no encourage results. The major problem was the fate of engrafted MSCs in vivo could not be defined. Some studies indicated that MSCs could induce immune response and result in the damage and rejection of MSCs. As toll like receptors (TLRs) are important in inducing of immune responses, in this study we study the role of TLR7 in mediating the immune status of MSCs isolated from umbilical cord. RESULTS: Our results indicated... |
| Tipo: Journal article |
Palavras-chave: Mesenchymal stem cells; Umbilical cord; Toll like receptor 7; Immunogenicity; Pro-inflammatory molecules. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100006 |
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